| Peer-Reviewed

Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria

Received: 25 November 2018     Accepted: 17 December 2018     Published: 22 January 2019
Views:       Downloads:
Abstract

The present study aimed to investigate the effect of the different types of solvents (water, ethanol and methanol) and extraction methods (ultrasound and maceration) on Punica granatum peels and to assess their antibacterial and antibiofilm activities. Our results revealed that the solvents and the extraction methods had a significant effect concerning the antimicrobial effects of P. granatum peels. The methanol extract produced by both the ultrasound and the maceration extraction techniques and the ethanol extract produced by the maceration extraction technique showed the most potent effect with the same bacteriostatic effect (MIC=25 mg/ml) on Staphylococcus epidermidis (CIP444). The antibacterial activities for all the P. granatum peels extracts were the same against E. coli (ATCC35218), with an MIC=25 mg/ml. The results showed that the methanol and the ethanol extracts produced by both the ultrasound and the maceration extraction techniques had an appreciable antibiofilm activity against S. epidermidis (CIP 444). These extracts showed the highest prevention capacity at a concentration of 12.5 mg/ml. In addition, the highest biofilm eradication activity was obtained using the ethanol extract with the ultrasound technique at a concentration of 25 mg/ml. We concluded that the P. granatum peels extracts have great potential as future natural antibacterial agents.

Published in Journal of Diseases and Medicinal Plants (Volume 4, Issue 6)
DOI 10.11648/j.jdmp.20180406.11
Page(s) 133-141
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Punica granatum Peels, Antibacterial Activity, Antibiofilm Activity

References
[1] Lansky EP, Newman RA. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J Ethnopharmacol. 2007; 109:177–206.
[2] Mena P, Vegara S, Martí N, García-Viguera C, Saura D, Valero M. Changes on indigenous microbiota, colour, bioactive compounds and antioxidant activity of pasteurised pomegranate juice. Food Chem. 2003:141:2122–2129.
[3] Seeram NP, Schulmann NR, Heber D. Pomegranates: AncientRoots to Modern Medicine. Boca Raton, USA: CRC Press. 2006.
[4] Naqvi SA, Khan MS, Vohora SB. Antibacterial, antifungal and anthelmintic investigations on Indian medicinal plants. Fitoterapia. 1991; 62:221–228.
[5] Zaid H, Saad B. State of the Art of Diabetes Treatment in Greco-Arab and Islamic Medicine. Bioactive Food as Dietary Interventions for Diabetes. 2013; 327–337.
[6] Kim YH, Choi EM. Stimulation of osteoblastic differentiation and inhibition of interleukin-6 and nitric oxide in MC3T3-E1 cells by pomegranate ethanol extract. Phytother Res. 2009; 23:737–739.
[7] Jain S, Puri H. Ethnomedicinal plants of Jaunsar-Bawar Hills, Uttar Pradesh, India. J Ethnopharmacol. 1984; 12:213–222.
[8] Siang ST. Use of combined traditional Chinese and western medicine in the management of burns. Panminerva Med. 1983; 25:197–202.
[9] Singh VP, Sharma SK., Khare VS. Medicinal plants from Ujjain District Madhya Prades (Vol. 5). Part II. Indian Drugs and Pharmaceuticals. 1980.
[10] Arseculeratne SN, Gunatilaka A, Panabokke RG. Studies on medicinal plants of srilanka. part 14: Toxicity of some traditional medicinal herbs. J Ethnopharmacol. 1985; 13:323–335.
[11] Ismail T, Sestili P, Akhtar S. Pomegranate peel and fruit extracts: A review of potential anti-inflammatory and anti-infective effects. J Ethnopharmacol. 2012; 143:397–405.
[12] El-Nemr SE, Ismail IA, Ragab M. Chemical composition of juice and seeds of pomegranate fruit. Food / Nahrung. 1990; 34:601–606.
[13] Seeram NP, Aviram M, Zhang Y, Henning SM, Feng L, Dreher M, Heber D. Comparison of Antioxidant Potency of Commonly Consumed Polyphenol-Rich Beverages in the United States. J Agric Food Chem. 2008; 56:1415–1422.
[14] Seeram NP, Lee R, Heber D. Bioavailability of ellagic acid in human plasma after consumption of ellagitannins from pomegranate (Punicagranatum L.) juice. ClinChimActa. 2004; 348: 63-68.
[15] Negi P, Jayaprakasha G, Jena B. Antioxidant and antimutagenic activities of pomegranate peel extracts. Food Chem. 2003; 80:393–397.
[16] Zahin M, Aqil F, Ahmad I. Broad spectrum antimutagenic activity of antioxidant active fraction of Punicagranatum L. peel extracts. Mutat Res/Genet Toxicol Environ Mutagen. 2010; 703:99–107.
[17] Pai V, Chanu TR, Chakraborty R, Raju B, Lobo R, Ballal M. Evaluation of the antimicrobialactivity of Punica granatum peel against the entericpathogens: An in vitro study. Asian Journal of Plant Science and Research. 2011; 57:1–2.
[18] Taguri T, Tanaka T, Kouno I. Antimicrobial Activity of 10 Different Plant Polyphenols against Bacteria Causing Food-Borne Disease. Biol Pharm Bull. 2004; 27:1965–1969.
[19] Zeidan S, Hijazi A, Rammal H, Kobaissi A, Badran B. Extraction of Phenolic Compounds and Flavonoids From Eryngiumcreticum L. by Conventional and Non-conventional Extraction Techniques. World J Pharm and Pharmaceutical Sci. 2014; 3:1889–1898.
[20] Bandar H, Hijazi A, Rammal H, Hachem A, Saad Z, Badran B. Techniques for the Extraction of Bioactive Compounds fromLebaneseUrticadioica. Am J PhytomedicineClinTher. 2013; 1:507–513.
[21] Cockerill FR. Methods for dilution antimicrobialsusceptibility tests for bacteriathatgrowaerobically: Approved standard - ninthedition. Wayne, PA: CLSI. 2012.
[22] Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, Beachay E. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: A quantitative model for the adherence of staphylococci to medical devices. J ClinMicrobiol. 1985; 22:996–1006.
[23] Chokr A, Watier D, Eleaume H, Pangon B, Ghnassia J, Mack D, Jabbouri S. Correlation between biofilm formation and production of polysaccharide intercellular adhesin in clinical isolates of coagulase-negative staphylococci. Int J Med Microbiol. 2006; 296:381–388.
[24] El-Sherbini GM, Ibrahim KM, El-Sherbiny ET, Abdel-Hady NM, Morsy TA. Efficacy of Punicagranatum extract on in-vitro and in-vivo control of Trichomonasvaginalis. J Egypt SocParasitol. 2010; 40:229–244.
[25] Voravuthikunchai SP, Limsuwan S. Medicinal Plant Extracts as Anti–Escherichia coli O157:H7 Agents and Their Effects on Bacterial Cell Aggregation. J Food Prot. 2006; 69:2336–2341.
[26] Braga L, Shupp J, Cummings C, Jett M, Takahashi J, Carmo, L, Chartone E, Nascimento A. Pomegranate extract inhibits Staphylococcus aureus growth and subsequent enterotoxin production. J Ethnopharmacol. 2005; 96:335–339.
[27] Al-Zoreky N. Antimicrobial activity of pomegranate (Punicagranatum L.) fruit peels. International J Food Microbiol. 2009; 134:244–248.
[28] Burapadaja S, Bunchoo A. AntimicrobialActivity of Tannins fromTerminaliacitrina. Planta Med. 1995; 61:365–366.
[29] Dahham SS, Ali MN, Tabassum H, Khan M. Studies on antibacterial and antifungal activity of pomegranate (Punicagranatum L.). Am-Eurasian J Agric Environ Sci. 2010; 9:273–281.
[30] Adams LS, Seeram NP, Aggarwal BB, Takada Y, Sand D, Heber D. Pomegranate Juice, Total Pomegranate Ellagitannins, and Punicalagin Suppress Inflammatory Cell Signaling in Colon Cancer Cells. J Agric Food Chem. 2006; 54:980–985.
[31] Glazer I, Masaphy S, Marciano P, Bar-Ilan I, Holland D, Kerem Z, Amir R. Partial Identification of Antifungal Compounds from Punicagranatum Peel Extracts. J Agric Food Chem. 2012; 60:4841–4848.
[32] Liu Y, Gallardo-Moreno AM, Pinzon-Arango PA, Reynolds Y, Rodriguez G, Camesano TA. Cranberry changes the physicochemical surface properties of E. coli and adhesionwithuroepithelialcells. Colloids and Surfaces B: Biointerfaces. 2008; 65:35–42.
[33] Bate-Smith E. Haemanalysis of tannins: The concept of relative astringency. Phytochemistry. 1973; 12:907–912.
[34] Endo EH, Ueda-Nakamura T, Nakamura CV, Filho BP. Activity of Spray-dried Microparticles Containing Pomegranate Peel Extract against Candida albicans. Molecules. 2012; 17:10094-10107.
[35] Haslam E. Natural Polyphenols (Vegetable Tannins) as Drugs: Possible Modes of Action. J Nat Prod. 1996; 59:205–215.
[36] Machado T, Pinto A, Pinto M, Leal I, Silva M, Amaral A, Kuster R, Netto-Dossantos K. In vitro activity of Brazilian medicinal plants, naturally occurring naphthoquinones and their analogues, against methicillin-resistant Staphylococcus aureus. Int J Antimicrob Agents. 2003; 21:279–284.
[37] Naz S, Ahmad S, Ajaz Rasool S, Asad Sayeed S, Siddiqi R. Antibacterial activity directed isolation of compounds from Onosma hispidum. Microbiol Res. 2006; 161:43–48.
[38] Vasconcelos LC, Sampaio MC, Sampaio FC, Higino JS. Use of Punicagranatum as an antifungal agent againstcandidosisassociatedwith denture stomatitis. VerwendungvonPunicagranatumalsAntimykotikumgegenCandidose in VerbindungmitZahnprothesen-Stomatitis. Mycoses. 2003; 46:192–196.
[39] Voravuthikunchai SP, Sririrak T, Limsuwan S, Supawita T, Iida T, Honda T. Inhibitory Effects of Active Compounds from Punicagranatum Pericarp on Verocytotoxin Production by Enterohemorrhagic Escherichia coli O157: H7. J Heal Sci. 2005; 51:590–596.
[40] Bakkiyaraj D, Nandhini JR, Malathy B, Pandian SK. The anti-biofilm potential of pomegranate (PunicagranatumL.) extractagainsthumanbacterial and fungalpathogens. Biofouling. 2013; 29:929-937.
[41] Nuamsetti T, Dechayuenyong P, Tantipaibulvut S. Antibacterial activity of pomegranate fruit peels and arils. ScienceAsia. 2012; 38:319.
Cite This Article
  • APA Style

    Salam Nasreddine, Amale Mcheik, Mohammad Abdalla Al-Seblani, Hawraa Shahrour, Mariam Hammoud, et al. (2019). Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria. Journal of Diseases and Medicinal Plants, 4(6), 133-141. https://doi.org/10.11648/j.jdmp.20180406.11

    Copy | Download

    ACS Style

    Salam Nasreddine; Amale Mcheik; Mohammad Abdalla Al-Seblani; Hawraa Shahrour; Mariam Hammoud, et al. Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria. J. Dis. Med. Plants 2019, 4(6), 133-141. doi: 10.11648/j.jdmp.20180406.11

    Copy | Download

    AMA Style

    Salam Nasreddine, Amale Mcheik, Mohammad Abdalla Al-Seblani, Hawraa Shahrour, Mariam Hammoud, et al. Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria. J Dis Med Plants. 2019;4(6):133-141. doi: 10.11648/j.jdmp.20180406.11

    Copy | Download

  • @article{10.11648/j.jdmp.20180406.11,
      author = {Salam Nasreddine and Amale Mcheik and Mohammad Abdalla Al-Seblani and Hawraa Shahrour and Mariam Hammoud and Ali Chokr},
      title = {Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria},
      journal = {Journal of Diseases and Medicinal Plants},
      volume = {4},
      number = {6},
      pages = {133-141},
      doi = {10.11648/j.jdmp.20180406.11},
      url = {https://doi.org/10.11648/j.jdmp.20180406.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20180406.11},
      abstract = {The present study aimed to investigate the effect of the different types of solvents (water, ethanol and methanol) and extraction methods (ultrasound and maceration) on Punica granatum peels and to assess their antibacterial and antibiofilm activities. Our results revealed that the solvents and the extraction methods had a significant effect concerning the antimicrobial effects of P. granatum peels. The methanol extract produced by both the ultrasound and the maceration extraction techniques and the ethanol extract produced by the maceration extraction technique showed the most potent effect with the same bacteriostatic effect (MIC=25 mg/ml) on Staphylococcus epidermidis (CIP444). The antibacterial activities for all the P. granatum peels extracts were the same against E. coli (ATCC35218), with an MIC=25 mg/ml. The results showed that the methanol and the ethanol extracts produced by both the ultrasound and the maceration extraction techniques had an appreciable antibiofilm activity against S. epidermidis (CIP 444). These extracts showed the highest prevention capacity at a concentration of 12.5 mg/ml. In addition, the highest biofilm eradication activity was obtained using the ethanol extract with the ultrasound technique at a concentration of 25 mg/ml. We concluded that the P. granatum peels extracts have great potential as future natural antibacterial agents.},
     year = {2019}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria
    AU  - Salam Nasreddine
    AU  - Amale Mcheik
    AU  - Mohammad Abdalla Al-Seblani
    AU  - Hawraa Shahrour
    AU  - Mariam Hammoud
    AU  - Ali Chokr
    Y1  - 2019/01/22
    PY  - 2019
    N1  - https://doi.org/10.11648/j.jdmp.20180406.11
    DO  - 10.11648/j.jdmp.20180406.11
    T2  - Journal of Diseases and Medicinal Plants
    JF  - Journal of Diseases and Medicinal Plants
    JO  - Journal of Diseases and Medicinal Plants
    SP  - 133
    EP  - 141
    PB  - Science Publishing Group
    SN  - 2469-8210
    UR  - https://doi.org/10.11648/j.jdmp.20180406.11
    AB  - The present study aimed to investigate the effect of the different types of solvents (water, ethanol and methanol) and extraction methods (ultrasound and maceration) on Punica granatum peels and to assess their antibacterial and antibiofilm activities. Our results revealed that the solvents and the extraction methods had a significant effect concerning the antimicrobial effects of P. granatum peels. The methanol extract produced by both the ultrasound and the maceration extraction techniques and the ethanol extract produced by the maceration extraction technique showed the most potent effect with the same bacteriostatic effect (MIC=25 mg/ml) on Staphylococcus epidermidis (CIP444). The antibacterial activities for all the P. granatum peels extracts were the same against E. coli (ATCC35218), with an MIC=25 mg/ml. The results showed that the methanol and the ethanol extracts produced by both the ultrasound and the maceration extraction techniques had an appreciable antibiofilm activity against S. epidermidis (CIP 444). These extracts showed the highest prevention capacity at a concentration of 12.5 mg/ml. In addition, the highest biofilm eradication activity was obtained using the ethanol extract with the ultrasound technique at a concentration of 25 mg/ml. We concluded that the P. granatum peels extracts have great potential as future natural antibacterial agents.
    VL  - 4
    IS  - 6
    ER  - 

    Copy | Download

Author Information
  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon

  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon

  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon

  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon

  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon

  • Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadath Campus, Beirut, Lebanon

  • Sections